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il 1o mouse proteintech 60269 1 ig  (Proteintech)


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    Structured Review

    Proteintech il 1o mouse proteintech 60269 1 ig
    Il 1o Mouse Proteintech 60269 1 Ig, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 360 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+1o+mouse+proteintech+60269+1+ig/IL-10+Antibody/pmc07530053-170-92-94
    Average 96 stars, based on 360 article reviews
    il 1o mouse proteintech 60269 1 ig - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Biomarker Discovery:

    Article Title: Non-neuronal crosstalk promotes an inflammatory response in nodose ganglia cultures after exposure to byproducts from gram positive, high-fat-diet-associated gut bacteria
    Article Snippet: Slides were imaged on a Zeiss Axios Scope A1 Microscope (Zeiss, Jena, Germany) with a Zeiss Axiocam 305 color camera (Zeiss) in the University of Georgia’s microscopy core at 10 or 20x magnification. .. Antibodies selected are well-referenced in the literature (except for SOX10; validation images are provided on the manufacturer’s website) and antibody specificity was confirmed by 1) double staining with other cell specific markers, and 2) omitting the primary antibody resulting in lack of staining. table ft1 table-wrap mode="anchored" t5 caption a7 Anti- Source and Cataglog # Dilution Reference -NeuN (rabbit) Cell Signaling D3S3I 1:500 [ 53 ] -Iba1 (rabbit) Waco 019–19741 1:500 [ 23 , 28 ] -GFAP (rabbit) Proteintech 16825–1-AP 1:500 [ 54 ] -IL-6 (mouse) Proteintech 66146–1-Ig 1:500 [ 55 ] -IL-1O (mouse) Proteintech 60269–1-Ig 1:500 [ 56 ] -CCL2 (MCP1, mouse) Proteintech 66272–1-Ig 1:400 [ 57 ] -CD206 (mouse) Proteintech 60143–1-lg 1:400 [ 58 , 59 ] -SOX10 (mouse) Proteintech 66786–1-Ig 1:500 Donkey anti-rabbit Alexa Fluor 488 Life Technologies A-21206 1:1000 Donkey anti-mouse Alexa Fluor 546 Life Technologies A-10036 1:1000 Open in a separate window Antibodies 2.3. .. RNA Extraction Immediately after cultures were collected, fixed, and washed, RNA was extracted from cells using the PureLinkTM FFPE RNA Extraction Kit (Invitrogen, Carlsbad, CA USA) according to the manufacturer’s protocol with a 30 min incubation in proteinase K. Extracted RNA was stored at −80° C. 2.4. cDNA After quantification by Nanodrop, the extracted RnA was used as the template for cDNA production with the SSIV or SSIV VILO cDNA kit (Thermo Fisher, Waltham, MA USA) following the manufacturer’s protocol. cDNA was stored at −80° C until use.

    Article Title: Non-neuronal crosstalk promotes an inflammatory response in nodose ganglia cultures after exposure to byproducts from gram positive, high-fat-diet-associated gut bacteria
    Article Snippet: .. Antibodies selected are well-referenced in the literature (except for SOX10; validation images are provided on the manufacturer’s website) and antibody specificity was confirmed by 1) double staining with other cell specific markers, and 2) omitting the primary antibody resulting in lack of staining. table ft1 table-wrap mode="anchored" t5 caption a7 Anti- Source and Cataglog # Dilution Reference -NeuN (rabbit) Cell Signaling D3S3I 1:500 [ 53 ] -Iba1 (rabbit) Waco 019–19741 1:500 [ 23 , 28 ] -GFAP (rabbit) Proteintech 16825–1-AP 1:500 [ 54 ] -IL-6 (mouse) Proteintech 66146–1-Ig 1:500 [ 55 ] -IL-1O (mouse) Proteintech 60269–1-Ig 1:500 [ 56 ] -CCL2 (MCP1, mouse) Proteintech 66272–1-Ig 1:400 [ 57 ] -CD206 (mouse) Proteintech 60143–1-lg 1:400 [ 58 , 59 ] -SOX10 (mouse) Proteintech 66786–1-Ig 1:500 Donkey anti-rabbit Alexa Fluor 488 Life Technologies A-21206 1:1000 Donkey anti-mouse Alexa Fluor 546 Life Technologies A-10036 1:1000 Open in a separate window Antibodies .. Immediately after cultures were collected, fixed, and washed, RNA was extracted from cells using the PureLinkTM FFPE RNA Extraction Kit (Invitrogen, Carlsbad, CA USA) according to the manufacturer’s protocol with a 30 min incubation in proteinase K. Extracted RNA was stored at −80° C.

    Double Staining:

    Article Title: Non-neuronal crosstalk promotes an inflammatory response in nodose ganglia cultures after exposure to byproducts from gram positive, high-fat-diet-associated gut bacteria
    Article Snippet: Slides were imaged on a Zeiss Axios Scope A1 Microscope (Zeiss, Jena, Germany) with a Zeiss Axiocam 305 color camera (Zeiss) in the University of Georgia’s microscopy core at 10 or 20x magnification. .. Antibodies selected are well-referenced in the literature (except for SOX10; validation images are provided on the manufacturer’s website) and antibody specificity was confirmed by 1) double staining with other cell specific markers, and 2) omitting the primary antibody resulting in lack of staining. table ft1 table-wrap mode="anchored" t5 caption a7 Anti- Source and Cataglog # Dilution Reference -NeuN (rabbit) Cell Signaling D3S3I 1:500 [ 53 ] -Iba1 (rabbit) Waco 019–19741 1:500 [ 23 , 28 ] -GFAP (rabbit) Proteintech 16825–1-AP 1:500 [ 54 ] -IL-6 (mouse) Proteintech 66146–1-Ig 1:500 [ 55 ] -IL-1O (mouse) Proteintech 60269–1-Ig 1:500 [ 56 ] -CCL2 (MCP1, mouse) Proteintech 66272–1-Ig 1:400 [ 57 ] -CD206 (mouse) Proteintech 60143–1-lg 1:400 [ 58 , 59 ] -SOX10 (mouse) Proteintech 66786–1-Ig 1:500 Donkey anti-rabbit Alexa Fluor 488 Life Technologies A-21206 1:1000 Donkey anti-mouse Alexa Fluor 546 Life Technologies A-10036 1:1000 Open in a separate window Antibodies 2.3. .. RNA Extraction Immediately after cultures were collected, fixed, and washed, RNA was extracted from cells using the PureLinkTM FFPE RNA Extraction Kit (Invitrogen, Carlsbad, CA USA) according to the manufacturer’s protocol with a 30 min incubation in proteinase K. Extracted RNA was stored at −80° C. 2.4. cDNA After quantification by Nanodrop, the extracted RnA was used as the template for cDNA production with the SSIV or SSIV VILO cDNA kit (Thermo Fisher, Waltham, MA USA) following the manufacturer’s protocol. cDNA was stored at −80° C until use.

    Article Title: Non-neuronal crosstalk promotes an inflammatory response in nodose ganglia cultures after exposure to byproducts from gram positive, high-fat-diet-associated gut bacteria
    Article Snippet: .. Antibodies selected are well-referenced in the literature (except for SOX10; validation images are provided on the manufacturer’s website) and antibody specificity was confirmed by 1) double staining with other cell specific markers, and 2) omitting the primary antibody resulting in lack of staining. table ft1 table-wrap mode="anchored" t5 caption a7 Anti- Source and Cataglog # Dilution Reference -NeuN (rabbit) Cell Signaling D3S3I 1:500 [ 53 ] -Iba1 (rabbit) Waco 019–19741 1:500 [ 23 , 28 ] -GFAP (rabbit) Proteintech 16825–1-AP 1:500 [ 54 ] -IL-6 (mouse) Proteintech 66146–1-Ig 1:500 [ 55 ] -IL-1O (mouse) Proteintech 60269–1-Ig 1:500 [ 56 ] -CCL2 (MCP1, mouse) Proteintech 66272–1-Ig 1:400 [ 57 ] -CD206 (mouse) Proteintech 60143–1-lg 1:400 [ 58 , 59 ] -SOX10 (mouse) Proteintech 66786–1-Ig 1:500 Donkey anti-rabbit Alexa Fluor 488 Life Technologies A-21206 1:1000 Donkey anti-mouse Alexa Fluor 546 Life Technologies A-10036 1:1000 Open in a separate window Antibodies .. Immediately after cultures were collected, fixed, and washed, RNA was extracted from cells using the PureLinkTM FFPE RNA Extraction Kit (Invitrogen, Carlsbad, CA USA) according to the manufacturer’s protocol with a 30 min incubation in proteinase K. Extracted RNA was stored at −80° C.

    Staining:

    Article Title: Non-neuronal crosstalk promotes an inflammatory response in nodose ganglia cultures after exposure to byproducts from gram positive, high-fat-diet-associated gut bacteria
    Article Snippet: Slides were imaged on a Zeiss Axios Scope A1 Microscope (Zeiss, Jena, Germany) with a Zeiss Axiocam 305 color camera (Zeiss) in the University of Georgia’s microscopy core at 10 or 20x magnification. .. Antibodies selected are well-referenced in the literature (except for SOX10; validation images are provided on the manufacturer’s website) and antibody specificity was confirmed by 1) double staining with other cell specific markers, and 2) omitting the primary antibody resulting in lack of staining. table ft1 table-wrap mode="anchored" t5 caption a7 Anti- Source and Cataglog # Dilution Reference -NeuN (rabbit) Cell Signaling D3S3I 1:500 [ 53 ] -Iba1 (rabbit) Waco 019–19741 1:500 [ 23 , 28 ] -GFAP (rabbit) Proteintech 16825–1-AP 1:500 [ 54 ] -IL-6 (mouse) Proteintech 66146–1-Ig 1:500 [ 55 ] -IL-1O (mouse) Proteintech 60269–1-Ig 1:500 [ 56 ] -CCL2 (MCP1, mouse) Proteintech 66272–1-Ig 1:400 [ 57 ] -CD206 (mouse) Proteintech 60143–1-lg 1:400 [ 58 , 59 ] -SOX10 (mouse) Proteintech 66786–1-Ig 1:500 Donkey anti-rabbit Alexa Fluor 488 Life Technologies A-21206 1:1000 Donkey anti-mouse Alexa Fluor 546 Life Technologies A-10036 1:1000 Open in a separate window Antibodies 2.3. .. RNA Extraction Immediately after cultures were collected, fixed, and washed, RNA was extracted from cells using the PureLinkTM FFPE RNA Extraction Kit (Invitrogen, Carlsbad, CA USA) according to the manufacturer’s protocol with a 30 min incubation in proteinase K. Extracted RNA was stored at −80° C. 2.4. cDNA After quantification by Nanodrop, the extracted RnA was used as the template for cDNA production with the SSIV or SSIV VILO cDNA kit (Thermo Fisher, Waltham, MA USA) following the manufacturer’s protocol. cDNA was stored at −80° C until use.

    Article Title: Non-neuronal crosstalk promotes an inflammatory response in nodose ganglia cultures after exposure to byproducts from gram positive, high-fat-diet-associated gut bacteria
    Article Snippet: .. Antibodies selected are well-referenced in the literature (except for SOX10; validation images are provided on the manufacturer’s website) and antibody specificity was confirmed by 1) double staining with other cell specific markers, and 2) omitting the primary antibody resulting in lack of staining. table ft1 table-wrap mode="anchored" t5 caption a7 Anti- Source and Cataglog # Dilution Reference -NeuN (rabbit) Cell Signaling D3S3I 1:500 [ 53 ] -Iba1 (rabbit) Waco 019–19741 1:500 [ 23 , 28 ] -GFAP (rabbit) Proteintech 16825–1-AP 1:500 [ 54 ] -IL-6 (mouse) Proteintech 66146–1-Ig 1:500 [ 55 ] -IL-1O (mouse) Proteintech 60269–1-Ig 1:500 [ 56 ] -CCL2 (MCP1, mouse) Proteintech 66272–1-Ig 1:400 [ 57 ] -CD206 (mouse) Proteintech 60143–1-lg 1:400 [ 58 , 59 ] -SOX10 (mouse) Proteintech 66786–1-Ig 1:500 Donkey anti-rabbit Alexa Fluor 488 Life Technologies A-21206 1:1000 Donkey anti-mouse Alexa Fluor 546 Life Technologies A-10036 1:1000 Open in a separate window Antibodies .. Immediately after cultures were collected, fixed, and washed, RNA was extracted from cells using the PureLinkTM FFPE RNA Extraction Kit (Invitrogen, Carlsbad, CA USA) according to the manufacturer’s protocol with a 30 min incubation in proteinase K. Extracted RNA was stored at −80° C.



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    il 1o mouse proteintech 60269 1 ig  (Proteintech)
    96
    Proteintech il 1o mouse proteintech 60269 1 ig
    Il 1o Mouse Proteintech 60269 1 Ig, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+1o+mouse+proteintech+60269+1+ig/IL-10+Antibody/pmc07530053-170-92-94
    Average 96 stars, based on 1 article reviews
    il 1o mouse proteintech 60269 1 ig - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier

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